SR EN ISO 9308-2:2014
Calitatea apei. Numărarea de Escherichia coli şi de bacterii coliforme. Partea 2: Metoda numărului cel mai probabil
ISO 9308-2:2012 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the "Most Probable Number" (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However it must not be used for the enumeration of coliform bacteria in marine water. When using for the enumeration of E. coli in marine waters, a 1›10 dilution in sterile water is typically required, although the method has been shown to work well with some marine waters that have a lower than normal concentration of salts. In the absence of data to support the use of the method without dilution, a 1›10 dilution is used. This method relies upon the detection of E. coli based upon expression of the enzyme b-D-glucuronidase and consequently does not detect many of the enterohaemorhagic strains of E. coli, which do not typically express this enzyme.
Data aprobării : 30.09.2014
Număr de pagini : 58
ICS : 07.100.20 Microbiologia apei
Comitet tehnic : 52 - Calitatea apei